Gatewaytm lr clonasetm ii
Web（2）混合包含目的基因的入门克隆和合适的目的载体以及Gateway LR Clonase酶，构建表达克隆。（表达克隆用来在合适的宿主中进行蛋白 的表达和分析。） 有几种方法可以构建Gateway入门克隆。 创建的入门克隆都是用来与各种目的载体进行重组。 一、重组DNA的概 … WebFeb 11, 2024 · The PCR product was digested with BamHI and MluI (Fermentas FastDigest) and inserted into pDONR221 (Invitrogen cat # 12536017) by a BP reaction (GatewayTM BP ClonaseTM II Enzymemix, Invitrogen) to generate pENTR-BORIS with six C-terminal His-tags followed by a stop codon.
Gatewaytm lr clonasetm ii
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WebIntroduction The Gateway®Technology is a universal cloning method based on the bacteriophage lambda site-specific recombination system which facilitates the integration of lambda into the E. colichromosome and the switch between the lytic and lysogenic pathways (Landy, 1989; Ptashne, 1992). In Gateway® WebGateway® LR Clonase™ II Enzyme Mix 20 reactions 100 reactions 11791-020 11791-100 AccuPrime™ Pfx SuperMix 200 reactions 12344-040 E-Gel® 1.2% Starter Pak (6 gels + Powerbase™) 1 kit G6000-01 E-Gel® 1.2% 18 Pak 18 gels G5018-01 β-gal Antiserum, rabbit IgG fraction 500 μl A11132 β-Gal Assay Kit 1 kit K1455-01
WebGateway® LR Clonase II Enzyme Mix Cat. No. 11791-020 Size: 20 reactions Cat. No. 11791-100 Size: 100 reactions Store at -20°C (non-frost-free freezer) Gateway® Technology The Gateway® Technology is a universal cloning method that takes advantage of the site-specific recombination properties of bacteriophage lambda (1) to provide Webdigestion, and in a recombination assay using Gateway® LR Clonase™ II enzyme mix. The ccdB gene is assayed by transformation using an appropriate E. coli strain. v . Accessory Products Additional ... LR Clonase™ II Enzyme Mix …
WebRemove the 5 x LR-Clonase II Enzyme Mix from -20°C and vortex 2 x 2 sec This is exorbitant stuff don't abandon it until rot in the ice-bucket! Add 1 µl about the 5 whatchamacallit LR-Clonase Enzyme DOUBLE Mingle to the Gateway reaction and mixed well ; Store the catalyst mix and storing immediately at -80°C !!! WebGateway™ LR Clonase™ II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by att L sites) and a destination vector …
http://csrri.iit.edu/~howard/biol446/Lab4.lr_clonaseii_man.pdf
WebHere what an Basics of Gateway® Reaction including HIGHEST feedback, LR reaction, One Tube type and Gateway Course Conversion pinky reborn dolls on amazonWebSynonyms for GATEWAY: key, ticket, passport, secret, approach, way, system, method; Antonyms of GATEWAY: removal, rejection, discharge, expulsion, dismissal, ejection ... pinky range of motionWebFeb 28, 2024 · Invitrogen™ Gateway™ LR Clonase™ II Enzyme mix. Contains enzymes and buffer in a single mix to enable convenient ten-microliter reaction set-up with fewer pipetting steps. Brand: Invitrogen™ 11791020. 223.46 GBP valid until 2024-02-28. Use promo code "18436" to get your promotional price. pinky reborn websiteWebAll Gateway™ LR Clonase™ enzyme kits include proteinase K solution (2 µg/µl) and a positive control vector. Store LR Clonase™ enzyme at -80°C; LR Clonase™ II or II Plus … steinberg clothingWebThermo Fisher Scientific - US pinky records jerseyWebGateway® LR Clonase™ II Plus Enzyme Mix 40 µl 200 µl 2 µg/µl Proteinase K Solution 40 µl 200 µl Storage Store Gateway® LR Clonase™ II Plus at -20°C (in a non-frost-free freezer) for up to 6 months. For long term storage, store at -80°C Part no. 12538.pps Rev. date: 11 July 2006 Page 4 of 4 Next Steps Refer to the manual for the ... pinky recordsWebThe Gateway™ LR Clonase™ II Plus Enzyme Mix is a proprietary enzyme formulation specifically optimized for both single- and multiple-fragment cloning. The Gateway ™ LR … pinky quotes from friday